Q:  使用碱性磷酸酶染色试剂盒（40749ES60），细胞，本身就是高表达碱性磷酸酶，做了处理之后可能就稍微低表达一些，拍出来的图，是细胞一整个轮廓是染色的？

A: 颜色深浅可以反应碱性磷酸酶的多少，但是微量差别可能肉眼无法观察，可以进行颜色灰度值测定（此方法可能会存在结果不稳定问题），可参考 WB 条带的计算方法。

Q:这个可以染胚胎干细胞吗？

A: 根据说明书中是可以的。本试剂盒可用于细胞或组织的碱性磷酸酶显色，包括诱导多功能干细胞iPS 的鉴定，也可以用于结合有碱性磷酸酶的膜的 Western 显色检测等。

Q:这个可以用于判断细胞活性不？

A: 不能用于细胞活性检测，本试剂盒主要用于细胞或组织的碱性磷酸酯酶显色包括诱导多功能干细胞 iPS 的鉴定，也可以用于 Western 等结合有碱性磷酸酯酶的膜的显色检测。同时也可以用于细胞或组织内源性的碱性磷酸酯酶显色。 检测细胞活性老师可以用cck-8 等试剂盒。

Q: 使用 24 孔板做成骨细胞分化后的碱性磷酸酶染色，请问可以使用本产品吗？

A: 可以的。

Q: 配好的碱性磷酸酶工作液可以重复是使用么，工作液如何保存，保存时间多久？

A: BCIP/NBT 染色工作液需现配先用，不能重复使用。

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