ALCAM Monoclonal antibody
ALCAM Monoclonal Antibody for IF-P, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
IF-P, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
1F7D6
Cat No : 67768-1-Ig
Print datasheet
Synonyms
验证数据展示
经过测试的应用
| Positive WB detected in | HT-1080 cells, U2OS cells, A549 cells, LNCaP cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human breast cancer tissue |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67768-1-Ig targets ALCAM in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Applications | IF-P, IHC, WB, ELISA Application Description |
| Tested Reactivity | Human |
| Immunogen | ALCAM fusion protein Ag16892 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | activated leukocyte cell adhesion molecule |
| Synonyms | ALCAM, CD166, CD166 antigen, FLJ38514, MEMD |
| Calculated Molecular Weight | 583 aa, 65 kDa |
| Observed Molecular Weight | 90-100 kDa |
| GenBank Accession Number | BC137097 |
| Gene Symbol | ALCAM |
| Gene ID (NCBI) | 214 |
| RRID | AB_2918534 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q13740 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues. Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ALCAM antibody 67768-1-Ig | Download protocol |
| IHC protocol for ALCAM antibody 67768-1-Ig | Download protocol |
| IF protocol for ALCAM antibody 67768-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |