Thioredoxin Polyclonal antibody

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

FC, IHC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Publications

Cat No : 14999-1-AP

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Synonyms

ADF, ATL derived factor, SASP, Thioredoxin, TRDX, TRX, TRX1, TXN

概述

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MSDS

WB analysis using 14999-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14999-1-AP (Thioredoxin antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 14999-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14999-1-AP (Thioredoxin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with TXN antibody included
IP experiment of HeLa using 14999-1-AP
IP result of anti-Thioredoxin (IP:14999-1-AP, 4ug; Detection:14999-1-AP 1:6000) with HeLa cells lysate 1520 ug.
FC experiment of HeLa using 14999-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Thioredoxin (14999-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
WB analysis of HeLa using 14999-1-AP
WB result of Thioredoxin antibody (14999-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Thioredoxin transfected HeLa cells.

Size

Positive WB detected in	HeLa cells, HepG2 cells, K562 cells, MCF-7 cells, Jurkat cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human liver cancer tissue, human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC detected in	HeLa cells

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:9000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Flow Cytometry (FC)	FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

14999-1-AP targets Thioredoxin in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications	FC, IHC, IP, WB, ELISA Application Description
Cited Applications	WB, IP, IF, IHC, CoIP, ELISA
Tested Reactivity	human
Cited Reactivity	human, mouse, rat, chicken
Immunogen	Thioredoxin fusion protein Ag6989 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	thioredoxin
Synonyms	ADF, ATL derived factor, SASP, Thioredoxin, TRDX, TRX, TRX1, TXN
Calculated Molecular Weight	12 kDa
Observed Molecular Weight	12 kDa
GenBank Accession Number	BC003377
Gene Symbol	TXN
Gene ID (NCBI)	7295
RRID	AB_2272597
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P10599
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Product Specific Protocols
WB protocol for Thioredoxin antibody 14999-1-AP	Download protocol
IHC protocol for Thioredoxin antibody 14999-1-AP	Download protocol
IP protocol for Thioredoxin antibody 14999-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
mouse	WB	Crit Care Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein. Authors - Lingbing Zhang View Article
mouse	WB	Acta Pharm Sin B Fluorine-thiol displacement probes for acetaminophen's hepatotoxicity Authors - Benjamin L Prather View Article
human	WB	Nat Commun Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents. Authors - Ziwen Wang View Article
		Nano Converg Detection of thioredoxin-1 using ultra-sensitive ELISA with enzyme-encapsulated human serum albumin nanoparticle. Authors - Myeong-Jun Lee View Article
mouse	WB	Cell Rep The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense. Authors - Wu Hongtan H View Article
mouse	WB	Theranostics TFE3, a potential therapeutic target for Spinal Cord Injury via augmenting autophagy flux and alleviating ER stress. Authors - Kailiang Zhou View Article