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- KD/KO Validated
CD63 Polyclonal antibody
CD63 Polyclonal Antibody for WB, IF/ICC,IF-P, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (6)
Applications
WB, IF/ICC,IF-P, IHC, ELISA and More (1)
Conjugate
Unconjugated
Publications
340
Cat No : 25682-1-AP
Print datasheet
Synonyms
验证数据展示
经过测试的应用
| Positive WB detected in | human urine exosomes tissue, human serum exosomes |
| Positive IHC detected in | human malignant melanoma tissue, human tonsillitis tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human malignant melanoma tissue, A431 cells |
Planning an IHC experiment? We recommend our IHCeasy CD63 Ready-To-Use IHC Kit. CD63 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 316 publications below |
| IHC | See 7 publications below |
| IF | See 15 publications below |
| IP | See 1 publications below |
| FC | See 2 publications below |
产品信息
25682-1-AP targets CD63 in WB, IP, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IF/ICC,IF-P, IHC, ELISA Application Description |
| Cited Applications | WB, IP, IF, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human, chicken, monkey, rabbit, zebrafish, pig, canine |
| Immunogen | CD63 fusion protein Ag19690 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | CD63 molecule |
| Synonyms | CD63, CD63 antigen, CD63 molecule, Granulophysin, ME491, MLA1, OMA81H, Tetraspanin 30, Tspan 30, TSPAN30 |
| Calculated Molecular Weight | 26 kDa |
| Observed Molecular Weight | 28-35 kDa |
| GenBank Accession Number | BC002349 |
| Gene Symbol | CD63 |
| Gene ID (NCBI) | 967 |
| RRID | AB_2783831 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P08962 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD63 is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediates signal transduction events that play a role in the regulation of cell development, activation, growth, and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it as a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD63 antibody 25682-1-AP | Download protocol |
| IHC protocol for CD63 antibody 25682-1-AP | Download protocol |
| IF protocol for CD63 antibody 25682-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
- All (340)
- KD/KO (1)
- WB (316)
- IHC (7)
- IF (15)
- IP (1)
- FC (2)
| Species | Application | Title |
|---|---|---|
Cell Res In vivo self-assembled small RNAs as a new generation of RNAi therapeutics. View Article | ||
ACS Nano Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration. View Article | ||
Adv Sci (Weinh) Astrocyte-Derived Extracellular Vesicular miR-143-3p Dampens Autophagic Degradation of Endothelial Adhesion Molecules and Promotes Neutrophil Transendothelial Migration after Acute Brain Injury View Article | ||
J Extracell Vesicles Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours. View Article | ||
Biosens Bioelectron Aptamer-bivalent-cholesterol-mediated proximity entropy-driven exosomal protein reporter for tumor diagnosis View Article | ||
Small Methods Stimuli-Mediated Specific Isolation of Exosomes from Blood Plasma for High-Throughput Profiling of Cancer Biomarkers. View Article |
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